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货号： bs-0215R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0215R
- 英文名称
- CD95/FAS
- 中文名称
- 载脂蛋白1抗体
- 别 名
- ALPS 1A; ALPS1A; APO 1; Apo-1; Apo 1 antigen; APO 1 cell surface antigen; Apo-1 antigen; APO1; Apo1 antigen; APO1 cell surface antigen; Apoptosis antigen 1; Apoptosis mediating surface antigen FAS; Apoptosis-mediating surface antigen FAS; APT 1; APT1; CD 95; CD 95 antigen; CD95; CD95 antigen; Delta Fas; Delta Fas/APO 1/CD95; Delta Fas/APO1/CD95; FAS 1; FAS 827dupA; Fas AMA; FAS; FAS Antigen; FAS1; FASLG receptor; FASTM; TNF receptor superfamily, member 6; TNFRSF 6; TNFRSF6; TNR6_HUMAN; Tumor necrosis factor receptor superfamily member 6.
- Specific References (1) | bs-0215R has been referenced in 1 publications.[IF=1.08] Zhou, Ke-Wen, et al. "Overexpression of CIRP may reduce testicular damage induced by cryptorchidism." Clinical & Investigative Medicine 32.2 (2009): E103-E111. Mouse.PubMed:19331798
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 细胞凋亡
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 40-50kDa
- 细胞定位
- 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from rat FAS:35-110/327 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
FAS is a receptor for TNFSF6/FASL. The adaptor molecule FADDrecruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Apoptosis or programmed-cell death is a physiological process essential for the normal development and maintenance of homeostasis in many organisms. This “cellular suicide” can be mediated by the Fas antigen (CD95, APO1), a cell-surface glycoprotein, 40-50kDa, that belongs to the nerve growth factor/tumor necrosis factor (TNF) receptor family. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). It is type I membrane protein. Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adaptor proteins Contains 1 death domain.
Function:
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Subunit:
Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD. Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B. Interacts with FADD.
Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2, 3, 4, 5, 6: Secreted.
Tissue Specificity:
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Post-translational modifications:
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
DISEASE:
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Similarity:
Contains 1 death domain.
Contains 3 TNFR-Cys repeats.
SWISS:
P25446
Gene ID:
246097
Database links:Entrez Gene: 355Human
Entrez Gene: 14102Mouse
Entrez Gene: 246097Rat
Omim: 134637Human
SwissProt: P25445Human
SwissProt: P25446Mouse
SwissProt: Q63199Rat
Unigene: 244139Human
Unigene: 1626Mouse
Unigene: 162521Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
FAS(也称作Apo-1;CD95;FasL receptor;TNFR)是一个含有死亡结构域的受体，是一型膜蛋白，属于TNF-R家族成员，具有诱导细胞凋亡的功能，广泛分布于许多不同类型的细胞，主要用于各种恶性肿瘤（包括：乳腺癌、肾细胞癌、胃癌、肺癌以及肝病等）的研究。分子量为：40-50kDa。
FAS的凋亡信号主要是通过与其胞浆区相关的死亡结构域蛋白FADD介导的。FAS与FasL结合后，FADD一方面通过C端的DD结合FAS, 另一方面通过N端的DED与Caspase-8 N端DED结合，通过Caspase-8诱导效应性Caspase 蛋白酶的激活，并最终导致细胞凋亡的发生。FAS主要表达于活化淋巴细胞、单核细胞、中性粒细胞和成纤维细胞等。
Fas又称作APO-1/CD95，属TNF受体家族。Fas基因编码产物为分子量45KD的跨膜蛋白，分布于胸腺细胞，激活的T和B淋巴细胞，巨噬细胞，肝、脾、肺、心、脑、肠、睾丸和卵巢细胞等。
Fas蛋白与Fas配体结合后，会激活caspase，导致靶细胞走向凋亡。
- 产品图片
Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fas Polyclonal Antibody, Unconjugated(bs-0215R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Fas Polyclonal Antibody, Unconjugated(bs-0215R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue).
Primary Antibody:Rabbit Anti-CD95FAS antibody(bs-0215R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-0215R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-0215R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.