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货号： bs-1106R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1106R
- 英文名称
- Cytokeratin 8
- 中文名称
- 细胞角蛋白8抗体
- 别 名
- card2; Cardiac autoantigen 2 120kD; CK 8; CK8; CK-8; ck8; Cyk 8; cyk8; CYKER; Cytokeratin endo A; Cytokeratin-8; Cytokeratin8; DreK8; EndoA; k0; CYK8; k2c8; K2C8_HUMAN; k8; Keratin 8; Keratin type ii cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; Keratin8; KO; Krt 2.8; Krt 8; krt8; KRT-8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8.
- Specific References (3) | bs-1106R has been referenced in 3 publications.[IF=0.50] Zhang, Yongli, et al. "Calcitonin is expressed in the submaxillary glands of rats." Bosnian Journal of Basic Medical Sciences 14.4 (2014): 35-39. Rat.PubMed:24579969[IF=3.15] Qian, Xian, Xiaolu Shi, and Hongyi Wang. "Effect of paeoniflorin on the calcium ion concentration in salivary gland cells using confocal laser scanning microscopy." Am J Transl Res 8.9 (2016): 3678-3688. IF(ICC) ; Rat.PubMed:27725850[IF=1.84] Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017). WB ; Chicken.PubMed:0
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 信号转导
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 53kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CK8:51-150/483
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012].
Function:
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Subunit:
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1.
Subcellular Location:
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Tissue Specificity:
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Post-translational modifications:
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
DISEASE:
Defects in KRT8 are a cause of cirrhosis (CIRRH) [MIM:215600].
Similarity:
Belongs to the intermediate filament family.
SWISS:
P05787
Gene ID:
3856
Database links:Entrez Gene: 3856 Human
Entrez Gene: 16691 Mouse
Entrez Gene: 25626 Rat
Omim: 148060 Human
SwissProt: P05787 Human
SwissProt: P11679 Mouse
SwissProt: Q10758 Rat
Unigene: 533782 Human
Unigene: 708445 Human
Unigene: 358618 Mouse
Unigene: 11083 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
结构蛋白（Structural Proteins）
上皮细胞胞质中的骨架蛋白除微丝（肌动蛋白）、微管蛋白外，主要是细胞角蛋白（cytokeratins，CK）。CK8也是上皮细胞的特征性标记物：CK8存在于某些正常腺上皮及其肿瘤，包括许多导管上皮和腺上皮，如结肠、胃、小肠、气管的上皮和尿路上皮。CK8主要用于腺癌和导管癌的诊断，鳞状细胞癌一般不表达CK8。
- 产品图片
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CK8 Polyclonal Antibody, Unconjugated(bs-1106R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Cytokeratin 8 antibody (bs-1106R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Cytokeratin 8 antibody (bs-1160R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.