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货号： bs-0624R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0624R
- 英文名称
- CD272
- 中文名称
- B和T淋巴细胞衰减蛋白抗体
- 别 名
- B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; BTLA; BTLA1; CD272 antigen; FLJ16065; MGC129743; BTLA_HUMAN; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; CD272.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 淋巴细胞 t-淋巴细胞 b-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 28kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human B and T-lymphocyte attenuator:221-289/289 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
B and T lymphocyte attenuator (BTLA), an immunoglobulin domain-containing glycoprotein with two immunoreceptor tyrosine-based inhibitory motifs. BTLA is not expressed by naive T cells, but it is induced during activation and remains expressed on T helper type 1 (T(H)1) but not T(H)2 cells. Crosslinking BTLA with antigen receptors induces its tyrosine phosphorylation and association with the Src homology domain 2 (SH2)-containing protein tyrosine phosphatases SHP-1 and SHP-2, and attenuates production of interleukin 2 (IL-2). BTLA-deficient T cells show increased proliferation, and BTLA-deficient mice have increased specific antibody responses and enhanced sensitivity to experimental autoimmune encephalomyelitis. B7x, a peripheral homolog of B7, is a ligand of BTLA. Thus, BTLA is a third inhibitory receptor on T lymphocytes with similarities to cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) and programmed death 1 (PD-1).
Function:
Lymphocyte inhibitory receptor which inhibits lymphocytes during immune response.
Subunit:
Interacts with tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. Interacts with TNFRSF14/HVEM.
Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential).
Post-translational modifications:
Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.
N-glycosylated.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
Q7Z6A9
Gene ID:
151888
Database links:Entrez Gene: 151888Human
Entrez Gene: 208154Mouse
Entrez Gene: 407756Rat
Omim: 607925Human
SwissProt: Q7Z6A9Human
SwissProt: Q7TSA3Mouse
SwissProt: Q6PNM1Rat
Unigene: 445162Human
Unigene: 38199Mouse
Unigene: 124474Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
BTLA对T细胞的活化、增殖起着重要的负调控作用，BTLA相应配体为TNFR超家族中的疱疹病毒入侵介质（HVEM）, 其表达于包括T细胞在内的多种免疫细胞表面。
有学者将他定为CD28的超级族成员，B、T淋巴细胞衰减子-CD272主要用于细胞信号传导方面的研究。
近来国外学者对BTLA用于抑制肿瘤方面的研究也有了新的进展，认为B、T淋巴细胞衰减子对肿瘤的生长有抑制作用，探索新的肿瘤免疫治疗有了新的途径， 封闭此途径有可能成为肿瘤免疫治疗的新靶点。
- 产品图片
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD272 (bs-0624R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 28 kD
Observed band size: 48 kD
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CD272/BTLA Polyclonal Antibody, Unconjugated(bs-0624R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Jurkat cells(blue).
Primary Antibody:Rabbit Anti- CD272 antibody(bs-0624R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0624R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.