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货号： bs-0528R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0528R
- 英文名称
- CD24
- 中文名称
- CD24抗体
- 别 名
- CD 24; CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24_HUMAN; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; Cd24; Cd24a; Ly-52.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 神经生物学 干细胞 细胞粘附分子 细胞表面分子 糖蛋白 t-淋巴细胞 b-淋巴细胞 骨髓细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 8kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD24 Propeptide:60-80/80
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and in many B cells. The encoded protein is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. [provided by RefSeq, Jul 2008].
Function:
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
Subcellular Location:
Cell membrane.
Tissue Specificity:
B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells.
Post-translational modifications:
Extensively O-glycosylated.
DISEASE:
Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface.
Similarity:
Belongs to the CD24 family.
SWISS:
P25063
Gene ID:
100133941
Database links:Entrez Gene: 100133941 Human
Entrez Gene: 12484 Mouse
Entrez Gene: 25145 Rat
Omim: 600074 Human
SwissProt: P25063 Human
SwissProt: P24807 Mouse
SwissProt: Q07490 Rat
Unigene: 644105 Human
Unigene: 721443 Human
Unigene: 29742 Mouse
Unigene: 6007 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经干细胞标志物
肿瘤标志物
CD24是一种低分子质量高度糖基化的黏附分子, 主要表达在造血系统. 最近研究表明, 在造血系统肿瘤和一些器官的实体瘤表面可见CD24高表达。
近几年很多学者认为：CD24在前列腺癌、非小细胞肺癌、鼻咽癌、原发性肝细胞癌、胆管细胞癌、麦克尔细胞癌、神经源性肿瘤、胰腺癌、胃癌、结肠癌、肾细胞癌、B细胞淋巴瘤、乳腺癌等肿瘤组织中表达增强, 且和肿瘤侵袭转移、复发和预后相关。
- 产品图片
Sample:
Colon carcinoma (Human) lysate at 30ug;
Heart(Rat) lysates, 30ug;
Primary: Anti-CD24 (bs-0528R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 8kD
Observed band size : 35kD
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD24 Polyclonal Antibody, Unconjugated(bs-0528R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: A549 (blue).
Primary Antibody:Rabbit Anti-CD24 antibody(bs-0528R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0528R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.