Anti-BMP2【科瑞奥博】

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Anti-BMP2

货号： bs-1012R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-1012R

英文名称: BMP2

中文名称: 骨形态发生蛋白2抗体

别名: bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2; BMP2A; BMP-2; BMP2_HUMAN.

: Specific References  (10)     |     bs-1012R has been referenced in 10 publications.
[IF=2.24] Wu, Mei, et al. "Expression analysis of BMP2, BMP5, BMP10 in human colon tissues from Hirschsprung disease patients." Int J Clin Exp Pathol 7.2 (2014): 529-536.  IHC-P ;  Human.
PubMed:n/a
[IF=1.72] Huang, Guo-fu, et al. "The Effect of Electroacupuncture on the Extracellular Matrix Synthesis and Degradation in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine 2014 (2014).  IHC-P ;  Rabbit.
PubMed:24987434
[IF=0.17] Yasuda, Hiromitsu, et al. "Osteogenic Effect of Local Administration of Fluvastatin using a Fluvastatin-gelatin Complex in Senile Osteoporosis Model Rat." Journal of Hard Tissue Biology 23.4 (2014): 389-398.  IHC-P ;  Rat.
PubMed:not posted yet
[IF=2.38] Zhu, Wenjing, et al. "Effect of PI3K/Akt Signaling Pathway on the Process of Prostate Cancer Metastasis to Bone." Cell Biochemistry and Biophysics (2015): 1-7.  WB ;  Human.
PubMed:22870197
[IF=8.38] Naeem, A. S., et al. "AKT1-mediated Lamin A/C degradation is required for nuclear degradation and normal epidermal terminal differentiation." Cell Death & Differentiation (2015).  WB ;  Rat.
PubMed:26045045
[IF=2.86] Kishi, Yuta, et al. "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway."International Journal of Molecular Sciences 16.10 (2015): 24820-24838.  WB ;  Mouse.
PubMed:26492236
[IF=5.58] Zhang, Jing, et al. "Combination of simvastatin, calcium silicate/gypsum, and gelatin and bone regeneration in rabbit calvarial defects." Scientific Reports 6 (2016): 23422.  IHC-P ;  Rabbit.
PubMed:26996657
[IF=1.82] Zhou, Zhixuan, et al. "CBX7 deficiency plays a positive role in dentin and alveolar bone development." Journal of Molecular Histology: 1-11.  WB ;  Mouse.
PubMed:27271093
[IF=0.70] Wu, Guoping, et al. "Gene Expression of Osteogenic Factors Following Gene Therapy in Mandibular Lengthening." Journal of Craniofacial Surgery26.2 (2015): 378-381.  IHC-P ;  Rabbit.
PubMed:25723654
[IF=0.00] Sari, Agnes Rosarina Prita, et al. "Characterization of ovine dermal papilla cell aggregation." International Journal of Trichology 8.3 (2016): 121.  IF(ICC) ;  Sheep.
PubMed:27625564

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 细胞生物免疫学发育生物学神经生物学生长因子和激素

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 13/44kDa

细胞定位: 分泌型蛋白

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human BMP2:283-365/396

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.

Function:
Induces cartilage and bone formation.

Subunit:
Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN.

Subcellular Location:
Secreted.

Tissue Specificity:
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Similarity:
Belongs to the TGF-beta family.

SWISS:
P12643

Gene ID:
650

Database links:

Entrez Gene: 650 Human

Entrez Gene: 12156 Mouse

Entrez Gene: 29373 Rat

Omim: 112261 Human

SwissProt: P12643 Human

SwissProt: P21274 Mouse

SwissProt: P49001 Rat

Unigene: 73853 Human

Unigene: 103205 Mouse

Unigene: 90931 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

神经干细胞标志物
BMP-2主要对未分化间充质细胞和骨系细胞起到募集和分化作用。在骨形成早期，BMP-2不仅可使未分化间质细胞向骨形成中心募集，并分化为骨系细胞，而且可使成纤维细胞、成肌细胞及骨髓的基细胞逆转分化为骨系细胞。其主要过程是：增加或抑制这些细胞内的某些特异性蛋白的分泌，使成纤维细胞分化为成骨细胞，成肌细胞快速分化为肥大的软骨细胞，并促进基质钙化。
对于成骨细胞，BMP-2则可使之维持其特有细胞表型，并诱导成骨细胞标志物的增高，促进细胞外基质钙化。在骨形成后期，BMP-2还作为一种破骨细胞分化因子与其它支持破骨细胞分化因子直接或间接刺激破骨细胞分化，参与骨的重建。

产品图片: Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- BMP2 (bs-1012R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 13 kD
Observed band size: 11 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BMP2) Polyclonal Antibody, Unconjugated (bs-1012R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat tibia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated(bs-1012R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining