Anti-Histone H4【科瑞奥博】

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Anti-Histone H4

货号： bs-23810R 基本售价： 1380.0 元规格： 100ul

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-23810R

英文名称: Histone H4

中文名称: 组蛋白H4抗体

别名: H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN; Osteogenic growth peptide; OGP.

规格价格: 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 100ul 200ul

研究领域

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 11kDa

细胞定位: 细胞核

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Histone H4:51-103/103

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008].

Function:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Subunit:
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

Subcellular Location:
Nucleus. Chromosome.

Post-translational modifications:
Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.

Similarity:
Belongs to the histone H4 family.

SWISS:
P62805

Gene ID:
121504

Database links:

Entrez Gene: 121504Human

Entrez Gene: 554313Human

Entrez Gene: 8294Human

Entrez Gene: 8359Human

Entrez Gene: 8360Human

Entrez Gene: 8361Human

Entrez Gene: 8362Human

Entrez Gene: 8363Human

Entrez Gene: 8364Human

Entrez Gene: 8365Human

Entrez Gene: 8366Human

Entrez Gene: 8367Human

Entrez Gene: 8368Human

Entrez Gene: 8370Human

Entrez Gene: 100041230Mouse

Entrez Gene: 100862646Mouse

Entrez Gene: 319155Mouse

Entrez Gene: 319156Mouse

Entrez Gene: 319157Mouse

Entrez Gene: 319158Mouse

Entrez Gene: 319159Mouse

Entrez Gene: 319160Mouse

Entrez Gene: 319161Mouse

Entrez Gene: 320332Mouse

Entrez Gene: 326619Mouse

Entrez Gene: 326620Mouse

Entrez Gene: 69386Mouse

Entrez Gene: 97122Mouse

GenBank: NM_003548Human

Omim: 142750Human

SwissProt: P02304Human

SwissProt: P62805Human

SwissProt: P02304Mouse

SwissProt: P62806Mouse

SwissProt: P02304Rat

Unigene: 143080Human

Unigene: 247816Human

Unigene: 248172Human

Unigene: 248178Human

Unigene: 248179Human

Unigene: 278483Human

Unigene: 352191Human

Unigene: 46423Human

Unigene: 528055Human

Unigene: 533295Human

Unigene: 55468Human

Unigene: 591790Human

Unigene: 655235Human

Unigene: 662174Human

Unigene: 706635Human

Unigene: 742244Human

Unigene: 14775Mouse

Unigene: 158272Mouse

Unigene: 227295Mouse

Unigene: 228709Mouse

Unigene: 246720Mouse

Unigene: 255646Mouse

Unigene: 260530Mouse

Unigene: 261642Mouse

Unigene: 261662Mouse

Unigene: 261664Mouse

Unigene: 377875Mouse

Unigene: 442307Mouse

Unigene: 486099Mouse

Unigene: 489077Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample:
NIH/3T3 cell(human) Lysate at 30 ug
Primary: Anti- Histone H4 (bs-23810R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13kD
Observed band size: 13 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4) Polyclonal Antibody, Unconjugated (bs-23810R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4) Polyclonal Antibody, Unconjugated (bs-23810R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H4) Polyclonal Antibody, Unconjugated (bs-23810R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.