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货号： bs-8853R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-8853R
- 英文名称
- phospho-Smad2/Smad3 (Thr8)
- 中文名称
- 磷酸化细胞信号转导分子SMAD2/SMAD3抗体
- 别 名
- Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phospho T8); P-Smad2 /3 (phospho T8); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 52kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8:PF(p-T)PP
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Function:
SMAD is a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.
Subcellular Location:
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
SWISS:
P84022
Gene ID:
4087
Database links:Entrez Gene: 4087Human
Entrez Gene: 4088Human
Entrez Gene: 17126Mouse
Entrez Gene: 17127Mouse
Entrez Gene: 25631Rat
Entrez Gene: 29357Rat
Omim: 601366Human
Omim: 603109Human
SwissProt: P84022Human
SwissProt: Q15796Human
SwissProt: Q62432Mouse
SwissProt: Q8BUN5Mouse
SwissProt: O70436Rat
SwissProt: P84025Rat
Unigene: 12253Human
Unigene: 714621Human
Unigene: 10636Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
cerebrum(mouse) Lysate at 40 ug
RSC96 cell(rat) Lysate at 30 ug
hepG2 cell(human) Lysate at 30 ug
Hcclm3 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 48,52 kD
Sample:
U251 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
Sample:
HUVEC cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
blank: A549 cells (blue line)
isotype control: rabbit IgG (orange line)
second antibody: goat anti-rabbit IgG (white blue line)
primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line); contration: 3μg /10^6 cells