Anti-TIM 3【科瑞奥博】

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Anti-TIM 3

货号： bs-8766R 基本售价： 1380.0 元规格： 100ul

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-8766R

英文名称: TIM 3

中文名称: T淋巴细胞膜蛋白3抗体

别名: TIM-3; HAVR2_MOUSE; Hepatitis A virus cellular receptor 2; Kidney injury molecule 3; KIM 3; KIM3; T cell immunoglobulin and mucin domain containing 3; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim 3; TIM-3; TIM3; TIMD-3; TIMD3.

规格价格: 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 100ul 200ul

研究领域: 细胞生物免疫学淋巴细胞 t-淋巴细胞细胞膜蛋白

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Mouse, Rat,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 29kDa

细胞定位: 细胞膜

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from mouse TIM 3:151-250/281 <Extracellular>

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]

Function:
Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.

Subcellular Location:
Membrane.

Tissue Specificity:
T-helper type 1 lymphocyte (Th1)-specific.

Post-translational modifications:
O-glycosylated with core 1 or possibly core 8 glycans.

Similarity:
Belongs to the immunoglobulin superfamily. TIM family.

Gene ID:
171285

Database links:

Entrez Gene: 171285Mouse

SwissProt: Q8VIM0Mouse

Unigene: 72168Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-TIM 3 (bs-8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated(bs-8766R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining