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货号： bs-0287R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：580.00元
- 规格：100ul
- 价格：920.00元
- 规格：200ul
- 价格：1600.00元
产品信息
- 产品编号
- bs-0287R
- 英文名称
- His tag
- 中文名称
- 聚组氨酸抗体标签抗体
- 别 名
- HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
- Specific References (5) | bs-0287R has been referenced in 5 publications.[IF=2.70] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013). WB ; Human.PubMed:N/A[IF=2.70] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012).PubMed:N/A[IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969 WB ; Virus.PubMed:N/A[IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013). WB ; Human.PubMed:23774531[IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126. IHC-P ; Mouse.PubMed:23198829
- 规格价格
- 50ul/580元购买 100ul/920元购买 200ul/1600元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 产品类型
- 标签抗体
- 研究领域
- 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 合成与降解 结合蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- fusionprotein,HHHHHHtagged
- 产品应用
- WB=1:500-2000 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- N/AkDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- Synthetic peptide: HHHHHH conjugated to KLH:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
SWISS:
N/A
Gene ID:
N/A
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
标签抗体
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。
2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括：
细菌，杆状病毒和哺乳动物）
4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌
5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫