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货号: bs-15463R 基本售价: 1380.0 元 规格: 100ul
- 规格:100ul
- 价格:1380.00元
- 规格:200ul
- 价格:2200.00元
产品信息
- 产品编号
- bs-15463R
- 英文名称
- HERC6
- 中文名称
- HERC6 E3泛素蛋白连接酶抗体
- 别 名
- EC 6.3.2.; FLJ20637; HECT domain and RCC1 like domain containing protein 6; HECT domain and RCC1-like domain-containing protein 6; Hect domain and RLD 6; HERC 6; HERC6; HERC6_HUMAN; Potential ubiquitin ligase; Probable E3 ubiquitin protein ligase HERC6; Probable E3 ubiquitin-protein ligase HERC6.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 115kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human HERC6:751-850/1022
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- Function:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity).
Subcellular Location:
Cytoplasm, cytosol.
Tissue Specificity:
Detected in brain, heart, placenta and testis.
Similarity:
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 5 RCC1 repeats.
SWISS:
Q8IVU3
Gene ID:
55008
Database links:Entrez Gene: 55008 Human
Entrez Gene: 362376 Rat
Omim: 609249 Human
SwissProt: Q8IVU3 Human
Unigene: 529317 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
- Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kDSample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti-HERC6 (bs-15463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115 kD
Observed band size: 115 kDSample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-HERC6(bs-15463R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 115kD
Observed band size: 115kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (bs-15463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.