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货号： bs-0608R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0608R
- 英文名称
- ICAM1/CD54
- 中文名称
- 细胞间粘附分子-1抗体
- 别 名
- BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor.
- Specific References (11) | bs-0608R has been referenced in 11 publications.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 干细胞 细胞膜受体 细胞粘附分子 细胞表面分子 糖蛋白 细胞类型标志物 t-淋巴细胞 b-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 56kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD54:201-300/537 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].
Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
ontains 5 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
P13597
Gene ID:
3383
Database links:Entrez Gene: 3383 Human
Entrez Gene: 15894 Mouse
Entrez Gene: 25464 Rat
Omim: 147840 Human
Unigene: 643447 Human
SwissProt: P05362 Human
SwissProt: P13597 Mouse
SwissProt: Q00238 Rat
Unigene: 435508 Mouse
Unigene: 12 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD54（ICAM-1）是分子量为60-110KD的膜型糖蛋白，表达于单核和内皮细胞。许多细胞如B或T淋巴细胞、胸腺细胞、纤维母细胞和上皮细胞都可诱导表达CD54，在调节免疫和炎症反应中CD54有重要作用。
- 产品图片
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO ICAM1 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD54 (bs-0608R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 (bs-0608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-0608R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): A431 cells(blue).
Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody (bs-0608R-PE-CY7)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
Blank control: HUVEC cells(blue).
Primary Antibody:Rabbit Anti-CD54 antibody(bs-0608R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (bs-0608R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.