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货号： bs-10009R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-10009R
- 英文名称
- E cadherin
- 中文名称
- 上皮钙粘附分子抗体
- 别 名
- E-cadherin; anion exchanger protein 3; Arc 1; Cadherin 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD 234; CD324; CD324 antigen; CDH1; CDHE; ECAD; Epithelial cadherin; epithelial calcium dependant adhesion protein; LCAM; Liver cell adhesion molecule; UVO; Uvomorulin.
- Specific References (5) | bs-10009R has been referenced in 5 publications.[IF=3.73] Chen, Cheng-Hsien, et al. "MicroRNA-328 Inhibits Renal Tubular Cell Epithelial-to-Mesenchymal Transition by Targeting the CD44 in Pressure-Induced Renal Fibrosis." PloS one 9.6 (2014): e99802. WB ; Rat.PubMed:24919189[IF=2.25] Neelam, Sudha, Morgan M. Brooks, and Patrick R. Cammarata. "Lenticular cytoprotection, part 2: Link between glycogen synthase kinase-3β, epithelial to mesenchymal transition, and mitochondrial depolarization." (2015) Molecular Vision. Human.PubMed:25593505[IF=7.43] Wang, Jing, et al. "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." BMC biology 15.1 (2017): 10. WB ; Human.PubMed:28193222[IF=3.23] Borin, Thaiz F., et al. "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." PLoS One 12.6 (2017). WB, IHC-P ; Mouse.PubMed:28609459[IF=3.13] Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017). WB ; Human.PubMed:0
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 发育生物学 信号转导 干细胞 转录调节因子 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 20/80kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human E-cadherin:401-500/882 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ∫-catenin, to regulate cadherin function.
Function:
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.
Subunit:
Homodimer.
Subcellular Location:
Cell junction. Cell membrane; Single-pass type I membrane protein.
Tissue Specificity:
Non-neural epithelial tissues.
Post-translational modifications:
Defects in CDH1 are the cause of hereditary diffuse gastric cancer (HDGC) . An autosomal dominant cancer predisposition syndrome with increased susceptibility to diffuse gastric cancer. Diffuse gastric cancer is a malignant disease characterized by poorly differentiated infiltrating lesions resulting in thickening of the stomach. Malignant tumors start in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. Note=Heterozygous germline mutations CDH1 are responsible for familial cases of diffuse gastric cancer. Somatic mutations in the has also been found in patients with sporadic diffuse gastric cancer and lobular breast cancer.
Similarity:
Contains 5 cadherin domains.
SWISS:
P12830
Gene ID:
999
Database links:Entrez Gene: 999Human
Entrez Gene: 12550Mouse
Entrez Gene: 83502Rat
Entrez Gene: 282637Cow
Entrez Gene: 442858Dog
Omim: 192090Human
SwissProt: Q6R8F2Cow
SwissProt: P12830Human
SwissProt: P09803Mouse
SwissProt: Q9R0T4Rat
Unigene: 461086Human
Unigene: 35605Mouse
Unigene: 1303Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti- E cadherin (bs-10009R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 20/80 kD
Observed band size: 80 kD
Sample:
HT29(Human) Cell Lysate at 30 ug
PC-3(Human) Cell Lysate at 30 ug
Primary: Anti-E cadherin (bs-10009R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20/80 kD
Observed band size: 130 kD
Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (bs-10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (bs-10009R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.