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货号： bs-8688R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-8688R
- 英文名称
- POD1
- 中文名称
- 足细胞表达蛋白/转录因子21抗体
- 别 名
- bHLHa23; Capsulin; Class A basic helix-loop-helix protein 23; Epicardin; POD 1; Pod-1; POD1; Podocyte expressed 1; Podocyte-expressed 1; TCF-21; TCF21; tcf21 ; TCF21_HUMAN; Transcription factor 21.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 心血管 细胞生物 信号转导 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 20 kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human TCF-21:41-410/179
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The basic helix-loop-helix (bHLH) class of transcription factors govern cell fate determination by controlling a variety of cellular differentiation processes. POD-1 (podocyte-expressed 1, also designated capsulin or epicardin) is a nuclear bHLH protein that is involved in the specification of select mesodermal cell populations associated with heart, cranial skeletal muscle, gut and urogenital system. POD-1 is selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine, pancreas and the epicardium, which gives rise to the coronary arteries. This epithelial-mesenchymal interaction is involved in the formation of numerous internal organs. POD-1 is also expressed in the mesothelium that gives rise to the spleen and in cells that give rise to smooth muscle. In addition to its role in kidney morphogenesis and spleen organogenesis, POD-1 may play a role in the development and sex determination of the mammalian gonad.
Function:
Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5-CANNTG-3).
Subcellular Location:
Nucleus.
Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
SWISS:
O43680
Gene ID:
6943
Database links:Entrez Gene: 421697 Chicken
Entrez Gene: 510873 Cow
Entrez Gene: 6943 Human
Entrez Gene: 21412 Mouse
Entrez Gene: 252856 Rat
Omim: 603306 Human
SwissProt: Q5E9S3 Cow
SwissProt: O43680 Human
SwissProt: O35437 Mouse
Unigene: 78061 Human
Unigene: 16497 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Kidney (Rat) Lysate at 40 ug
Primary: Anti-POD1 (bs-8688R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 23 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-POD1 (bs-8688R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 23 kD
Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- POD1 Polyclonal Antibody, Unconjugated(bs-8688R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-POD1 Polyclonal Antibody, Unconjugated(bs-8688R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining