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货号： bs-7112R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-7112R
- 英文名称
- EDG1
- 中文名称
- 内皮细胞分化鞘脂G蛋白偶联受体1抗体
- 别 名
- CD363; CHEDG1; D1S3362; ECGF1; EDG1; EDG 1; Endothelial differentiation G-protein coupled receptor 1; Endothelial differentiation sphingolipid G protein coupled receptor 1; G protein coupled sphingolipid receptor; S1P receptor 1; S1P receptor Edg-1; S1P1; S1PR1; S1PR1_HUMAN; Sphingosine 1 phosphate receptor Edg 1; Sphingosine 1 phosphate receptor EDG1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; Sphingosine 1phosphate receptor type 1 S1P1.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 信号转导 细胞凋亡 细胞粘附分子 G蛋白偶联受体 内皮细胞 细胞骨架
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 44kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human EDG1/CD363:51-150/382 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Sphingosine-1-phosphate receptor 1 (S1P receptor 1 or S1P1), also known as endothelial differentiation gene 1 (EDG1) is a protein that in humans is encoded by the S1PR1 gene. S1PR1 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P). S1PR1 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR1-5). S1PR1 was originally identified as an abundant transcript in endothelial cells and it has an important role in regulating endothelial cell cytoskeletal structure, migration, capillary-like network formation and vascular maturation. In addition, S1PR1 signaling is important in the regulation of lymphocyte maturation, migration and trafficking.
Function:
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. This inducible epithelial cell G-protein-coupled receptor may be involved in the processes that regulate the differentiation of endothelial cells. Seems to be coupled to the G(i) subclass of heteromeric G proteins.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Endothelial cells, and to a lesser extent, in vascular smooth muscle cells, fibroblasts, melanocytes, and cells of epithelioid origin.
Post-translational modifications:
S1P-induced endothelial cell migration requires the PKB/AKT1-mediated phosphorylation of the third intracellular loop at the Thr-236 residue.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P21453
Gene ID:
1901
Database links:Entrez Gene: 281135 Cow
Entrez Gene: 100050049 Horse
Entrez Gene: 1901 Human
Entrez Gene: 13609 Mouse
Entrez Gene: 29733 Rat
Omim: 601974 Human
SwissProt: Q5E9P3 Cow
SwissProt: P21453 Human
SwissProt: O08530 Mouse
SwissProt: P48303 Rat
Unigene: 154210 Human
Unigene: 982 Mouse
Unigene: 109455 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
研究发现，S1P1与VEGF肩并肩相互协作，从而促进血管生长。VEGF是很多不同抗癌药物的作用靶标。血管新生在很多疾病是异常的。靶向S1P1和VEGF可能要比只使用VEGF抑制剂更加有效地治疗疾病；S1P1是一种关键性的血管新生反应性基因。研究人员证实当新的血管网络形成时，由此产生的血液流动激活血管内皮细胞表面上的S1P1，并把信号传递到这些细胞内部从而让新形成的血管网络稳定化。因此阻断S1P1可能有助于切断给癌性肿瘤提供资源的血液供应。
- 产品图片
Sample:
H9C2 Cell (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti-EDG1 (bs-7112R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 50 kD
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti-EDG1 (bs-7112R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EDG1) Polyclonal Antibody, Unconjugated (bs-7112R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.