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货号： bs-6316R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6316R
- 英文名称
- Prostaglandin E Receptor EP1
- 中文名称
- 前列腺素EP1受体抗体
- 别 名
- Prostaglandin E Receptor EP1; EP1; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostaglandin E receptor 1 subtype EP1 42kDa; Prostaglandin E receptor 1 subtype EP1; Prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PE2R1_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 信号转导 转录调节因子 G蛋白偶联受体 G蛋白信号
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 42kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1:61-160/402 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P34995
Gene ID:
5731
Database links:Entrez Gene: 5731Human
Entrez Gene: 19216Mouse
Entrez Gene: 25637Rat
Omim: 176802Human
SwissProt: P34995Human
SwissProt: P35375Mouse
SwissProt: P70597Rat
Unigene: 159360Human
Unigene: 474430Mouse
Unigene: 11423Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Brain (Rat) Lysate at 40 ug
Primary: Anti-Prostaglandin E Receptor EP1 (bs-6316R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Prostaglandin E Receptor EP1 (bs-6316R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 45 kD
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PTGER1 Polyclonal Antibody, Unconjugated(bs-6316R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control (Black line): Mouse blood (Black).
Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (bs-6316R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.