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货号： bs-7052R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-7052R
- 英文名称
- Myosin light chain (phospho S20)
- 中文名称
- 磷酸化肌球蛋白调节多肽9(平滑肌亚型)抗体
- 别 名
- MYL9 (phospho S20); p-MLC(Ser20); phospho-MLC(Ser20); p-Myosin light chain(Ser20); MYL9_HUMAN; Myosin regulatory light polypeptide 9; 20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MLC2; MRLC1; MYRL2.
- Specific References (3) | bs-7052R has been referenced in 3 publications.[IF=3.82] Ikemura, S., et al. "Preventive effects of the anti-vasospasm agent via the regulation of the Rho-kinase pathway on the development of steroid-induced osteonecrosis in rabbits." Bone (2013). WB ; Rabbit.PubMed:23313282[IF=1.06] Li, Wei, et al. "Tanshinone II a protects against liopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway." Chinese Journal of Integrative Medicine 20 (2014): 216-223. WB ; Human.PubMed:24615214[IF=3.56] Hoj, Jacob P., et al. "Cellular contractility changes are sufficient to drive epithelial scattering." Experimental Cell Research (2014). WB ; Dog.PubMed:24780819
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 心血管 信号转导 干细胞 细胞骨架 细胞膜蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 20kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthesised phosphopeptide derived from human MYL9 around the phosphorylation site of Ser20:AT(p-S)NV
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues.
Subunit:
Myosin is an hexamer of 2 heavy chains and 4 light chains.
Tissue Specificity:
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Similarity:
Contains 3 EF-hand domains.
SWISS:
P24844
Gene ID:
10398
Database links:Entrez Gene: 10398 Human
Entrez Gene: 98932 Mouse
Entrez Gene: 296313 Rat
Omim: 609905 Human
SwissProt: P20689 Human
SwissProt: P24844 Human
SwissProt: Q9CQ19 Mouse
SwissProt: Q64122 Rat
Unigene: 504687 Human
Unigene: 271770 Mouse
Unigene: 228729 Rat
Unigene: 6870 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Myosin light chain (phospho S20) (bs-7052R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20 kD
Observed band size: 20 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(bs-7052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(bs-7052R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-MLC(Ser20) Polyclonal Antibody, Unconjugated(bs-7052R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei