| 货号 | PRD240-50 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | CEDLAP; DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta; TGF-beta-1; transforming growth factor beta-1; transforming growth factor, beta 1 | 全称 | Transforming Growth Factor beta 1 |
| 反应种属 | Human |
| 来源 | Chinese Hamster Ovary cell line, CHO-derived Ala279 - Ser390 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7040 (Human); 21803 (Mouse); 59086 (Rat); 397078 (Porcine); 100033900 (Equine) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is typically 0.04-0.2 ng/mL. |
| 溶解方法 | Add ProDot directly to the medium or reconstitute in the vial at 20 μg/mL in sterile 4 mM HCl. |
| 背景 | Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus. TGF-beta ligands are initially synthesized as precursor proteins that undergo proteolytic cleavage. The mature segments form active ligand dimers via a disulfide-rich core consisting of the characteristic cysteine knot. TGF-beta signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta RIII) and a type II serine/threonine kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a type I serine/threonine kinase receptor, either ALK-1 or TGF-beta RI (also called ALK-5). The activated type I receptor phosphorylates and activates Smad proteins that regulate transcription. Use of other signaling pathways that are Smad-independent allows for distinct actions observed in response to TGF-beta in different contexts. |
| Accession # | P01137 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Ala279 |
| 预期分子量 | 12.8 (monomer) kDa |
| SDS-PAGE | 12 kDa, reducing conditions, 24 kDa, non-reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human TGF-beta 1 wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized with silver staining, showing singlebands at 12 kDa and 24 kDa, respectively. | |
ProDots® Recombinant Human TGF-beta 1 inhibitsRecombinant Mouse IL-4 (R&D Systems, Catalog # 404-ML)induced proliferation in the HT-2 mouse T cell line. The ED50 istypically 0.04-0.2 ng/mL. |
