| 货号 | PRD251-50 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | FGF7; FGF-7; fibroblast growth factor 7HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor) | 全称 | Keratinocyte Growth Factor |
| 来源 | E. coli-derived Cys32 - Thr194, with an N-terminal Met |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2252 (Human); 403915 (Canine) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL. |
| 溶解方法 | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot |
| 背景 | KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R. |
| Accession # | Q6FGV5 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Met |
| 预期分子量 | 19 kDa |
| SDS-PAGE | |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
ProDots® Recombinant Human KGF/FGF-7 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is typically 6-60 ng/mL. | |
1 μg/lane of ProDots® Recombinant Human KGF/FGF-7 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa. |
