| 货号 | PRD1845-50 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS |
| 来源 | E. coli-derived Cys24 - Gly197 (Cys24lle-lle), with N-terminal Met |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6469 (Human); 20423 (Mouse) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is typically 0.1-0.4 μg/mL. |
| 溶解方法 | For a stock solution, reconstitute at 100 µg/mL in sterile PBS, or simply roll ProDot |
| 背景 | Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO). |
| Accession # | NP_000184 N-termi |
| 运输条件 | Blue Ice |
| 预期分子量 | 20 kDa |
| SDS-PAGE | 22 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 22 kDa. | |
ProDots®Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is typically 0.1-0.4 μg/mL. |
