| 货号 | PRD255-50 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor | 全称 | Stem Cell Factor |
| 反应种属 | Human |
| 来源 | E. coli-derived Glu26-Ala189, with an N-terminal Met |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 1-5 ng/mL. |
| 溶解方法 | Add ProDot directly to the medium or reconstitute in the vial at 50 μg/mL in sterile PBS. |
| 背景 | Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature human SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of human SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), human SCF shares 75 - 83% aa sequence identity with canine, feline, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF-R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels. |
| Accession # | P21583.1 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Met |
| 预期分子量 | 18.6 kDa |
| SDS-PAGE | 19 kDa, reducing conditions. |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human SCF wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized with silver staining, showing a singleband at 19 kDa. | |
ProDots® Recombinant Human SCF stimulates cellproliferation of the TF-1 human erythroleukemic cell line. The ED50for this effect is typically 1-5 ng/mL. |
