| 货号 | PRD314-10 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | BMP-2B; BMP2B1; BMP2BMCOPS6; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; OFC11; ZYME | 全称 | Bone Morphogenetic Protein 4 |
| 反应种属 | Human |
| 来源 | Mouse myeloma cell line, NS0-derived Ser293 - Arg408 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is typically 2.5-15.0 ng/mL. |
| 溶解方法 | Add ProDot directly to the medium or reconstitute at 50-200 μg/mL in sterile 4 mM HCl. |
| 背景 | BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype. Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks. |
| Accession # | Q53XC5 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Ser293 |
| 预期分子量 | 13 (monomer) kDa |
| SDS-PAGE | 22-25 kDa, reducing conditions 37-41, non-reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing multiple bands at 22-25 kDa and 37-41 kDa, respectively. Multiple bands in the gel are due to variable glycosylation. | |
ProDots® Recombinant Human BMP-4 induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is typically 2.5-15.0 ng/mL. |
