| 货号 | PRD308-10 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | FL; Flt3 ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine | 全称 | fms-like Tyrosine Kinase 3 Ligand |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 21 (baculovirus)-derived Thr27 - Pro185 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2323 (Human); 14256 (Mouse); 493796 (Feline) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50for this effect is typically 0.2-1 ng/mL. |
| 溶解方法 | Add ProDot directly to the medium or reconstitute in the vial at 100 μg/mL in sterile PBS. |
| 背景 | Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation. |
| Accession # | AAA17999.1 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Thr27 |
| 预期分子量 | 17.5 kDa |
| SDS-PAGE | 17-30 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human Flt-3 Ligandwas resolved with SDS-PAGE under reducing (R) conditions andvisualized with silver staining, showing major bands at 20-24 kDa.The multiple bands are due to variable glycosylation of the protein. | |
ProDots® Recombinant Human Flt-3 Ligand stimulatescell proliferation in the BaF3 mouse Pro-B cell line transfected withmouse Flt-3. The ED50 for this effect is typically 0.2-1 ng/mL. |
