| 货号 | PRD345-10 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | FGF10; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; KGF-2; produced by fibroblasts of urinary bladder lamina propria | 全称 | Fibroblast Growth Factor 10 |
| 来源 | E. coli-derived Cys37 - Ser208 & Gly41 - Ser208 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2255 (Human); 14165 (Mouse); 25443 (Rat) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 20-100 ng/mL. |
| 溶解方法 | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot |
| 背景 | FGF-10 (Fibroblast Growth Factor 10) is a heparin binding glycoprotein in the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer. |
| Accession # | O15520 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Cys37 & Gly41 |
| 预期分子量 | 19.5 kDa |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
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ProDots® Recombinant Human FGF-10 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is typically 20-100 ng/mL. | |
1 μg/lane of ProDots® Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa. |
