| 货号 | PRD220-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | PDGFBB; PDGF-BB | 全称 | Platelet-derived Growth Factor BB |
| 反应种属 | Human |
| 来源 | E. coli-derived Ser82 - Thr190 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5155 (Human); 24628 (Rat) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 1.5-6.0 ng/mL. |
| 溶解方法 | Add ProDot directly to medium or reconstitute at 100 µg/mL in sterile 4mM HCl. |
| 背景 | The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta). |
| Accession # | Q6FHE7 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Ser82 |
| 预期分子量 | 12.3 (monomer) kDa |
| SDS-PAGE | 12 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human PDF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively. | |
ProDots® Recombinant Human PDGF-BB stimulates cell proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is typically 1.5-6.0 ng/mL. |
