| 货号 | PRD1206-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | FGF3; FGF-3; fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); fibroblast growth factor 3; HBGF-3; Hst; INT-2; mouse; oncogene INT2 | 全称 | Fibroblast Growth Factor 3 |
| 来源 | E. coli-derived Asp28 - Arg212, with an N-terminal Met |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2248 (Human) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is typically 0.02-0.1 μg/mL in the presence of 1 μg/mL of heparin. |
| 溶解方法 | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot |
| 背景 | Fibroblast growth factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair. Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated. TheFGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration. Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site. Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus. FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity. FGF-3 has been implicated in the induction of inner ear development. Recent studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation. During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by sef (similar expression to FGF genes), a transmembrane protein that shares intracellular sequence similarities with the IL-17 receptor. |
| Accession # | NP_005238 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Met |
| 预期分子量 | 21.1 kDa |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
ProDots® Recombinant Human FGF-3 stimulated cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is typically 0.02-0.1 μg/mL. | |
1 μg/lane of ProDots® Recombinant Human FGF-3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa. |
