| 货号 | PRD4645-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1 | 全称 | Roof Plate-specific Spondin 1 |
| 来源 | Chinese Hamster Ovary cell line, CHO-derived Arg31 - Ala263 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 284654 (Human); 192199 (Mouse) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). |
| 溶解方法 | For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll ProDot |
| 背景 | R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/beta-catenin signaling regulators. R-Spondin 1 enhances canonical Wnt/beta-catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors. R-Spondin 1 functions in dorsal neural tube development as well as male and female germ cell development. It also induces bone formation, intestinal crypt cell proliferation, angiogenesis, and insulin secretion from pancreatic beta cells. |
| Accession # | Q2MKA7 N-terminal Se |
| 运输条件 | Blue Ice |
| 预期分子量 | 25.6 kDa |
| SDS-PAGE | 39 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human R-Spondin 1 was resolved with SDS-PAGEunder reducing (R) and non-reducing (NR) conditions and visualized with silverstaining, showing a single band at 39 kDa. | |
ProDots® Recombinant Human R-Spondin 1, in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay. |
