| 货号 | PRD449-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI | 全称 | Leukemia Inhibitory Factor |
| 来源 | E. coli-derived Pro25 - Phe203 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3976 (Human); 16878 (Mouse); 403449 (Canine) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells. The ED50 is 0.1-0.6 ng/mL. |
| 溶解方法 | For a stock solution, reconstitute at 0.2-1 mg/mL in sterile PBS. For immediate use, simply roll ProDot |
| 背景 | Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. The activities of LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R beta and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. |
| Accession # | P09056 N-terminal Se |
| 运输条件 | Blue Ice |
| 预期分子量 | 20 kDa |
| SDS-PAGE | 20 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Mouse LIF was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized with silverstaining, showing a single band at 20 kDa. | |
ProDots®Recombinant Mouse LIF induces IL-6 secretion in M1 mouse myeloid leukemiacells. The ED50 for thiseffect is typically 0.1-0.6 ng/mL. |
