| 货号 | PRD203-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | Hematopoietic growth factor; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor | 全称 | Interleukin 3 |
| 反应种属 | Human |
| 来源 | E. coli-derived Ala20 - Phe152, with and without an N-terminal Met
|
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3562 (Human); 16187 (Mouse); 24495 (Rat) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 0.02-0.1 ng/mL. |
| 背景 | Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells. |
| Accession # | Q6GS87 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Ala20 and Met |
| 预期分子量 | 15 kDa |
| SDS-PAGE | 14 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human IL-3 wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 14 kDa. | |
ProDots® Recombinant Human IL-3 stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is typically 0.02-0.1 ng/mL. |
