| 货号 | PRD236-01 |
| 简介 | Supplied as ready-to-use 5 ug protein balls |
| 别名 | beta-urogastrone; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone | 全称 | Epidermal Growth Factor |
| 反应种属 | Human |
| 来源 | E. coli-derived Asn971 - Arg1023, with an N-terminal Met |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1950 (Human); 13645 (Mouse); 25313 (Rat) |
| 内毒素水平 | <0.10 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is typically 20-100 pg/mL. |
| 背景 | EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration. |
| Accession # | P01133 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Met |
| 预期分子量 | 6 kDa |
| SDS-PAGE | 6 kDa, reducing conditions |
| 存放说明 | 4℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 |
|
1 μg/lane of ProDots® Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa. | |
ProDots® Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED50 for this effect is typically 20-100 pg/mL. |
