Recombinant Human GM-CSF, Animal-Free Protein (1 MG)【科瑞奥博】

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Recombinant Human GM-CSF, Animal-Free Protein (1 MG)

货号： AFL215-01M 基本售价： 55491.6 元规格： 1 mg

产品信息

概述
货号	AFL215-01M
别名	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
全称	Granulocyte Macrophage Growth Factor
反应种属	Human
来源	E. coli-derived Ala18-Glu144 Produced using non-animal reagents in an animal-free laboratory.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
内毒素水平	<1.0 EU per 1 μg of the protein by the LAL method.
生物活性	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is typically 6-30 pg/mL. The specific activity of Recombinant Human GM-CSF is approximately 1.5 x 10⁴ IU/μg, which is calibrated against human GM-CSF WHO International Standard (NIBSC code: 88/646).
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3 - 5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha -helices (8 - 12). Mature human GM-CSF shares 63%-70% amino acid sequence identity with canine, feline, porcine, and rat GM-CSF and 54% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (13, 14). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (15). Human GM-CSF is active on canine and feline cells but not on murine cells (16-18).
Accession #	CAA26822
运输条件	Blue Ice
N-terminal Sequence Analysis	Ala18
预期分子量	14 kDa
SDS-PAGE	13 kDa, reducing conditions
存放说明	-20℃
纯度	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
参考文献	Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. Cao, Y. (2007) J. Clin. Invest. 117:2362. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. Heuser, M. et al. (2007) Semin. Hematol. 44:148. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321. Kaushansky, K. et al. (1992) Biochemistry 31:1881. Diederichs, K. et al. (1991) Science 254:1779. Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250. Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360. Wong, G.G. et al. (1985) Science 228:810. Onetto-Pothier, N. et al. (1990) Blood 75:59. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483. Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.

参考图片

1 μg/lane of Animal-Free^TM Recombinant Human IL-4 (Catalog # AFL215) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Animal-Free^TM Recombinant Human GM-CSF (Catalog # AFL215) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED₅₀ for this effect is typically 6-30 pg/mL.