| 货号 | 9109-GH-01M |
| 简介 | Suitable for 8,000 (20 ug size) or 40,000 (100 ug size) RNase B (10 ug) deglycosylation reactions using our protocol |
| 别名 | PNGase F; PNGF | 全称 | Peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine Amidase F |
| 来源 | E. coli-derived Ala41-Asn354 with N-terminal Met and 6-His tag |
| 产品组分 |
| 供应商 | R&D Systems |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions. |
| 背景 | PNGase F, peptide N-glycosidase F from Flavobacterium meningosepticum, catalyzes the hydrolysis of asparagine-linked high mannose, as well as hybrid and complex oligosaccharides from glycoproteins (1). Unlike glycosidases that hydrolyze glycosidic bonds, PNGase F is an amidase that cleaves the beta-aspartylglucosamine bond between the innermost GlcNAc of N-glycans and asparagine residues of glycoproteins (2). The enzyme is highly active on various N-glycans except those with the innermost GlcNAc modified with alpha 1-3-linked core fucose, which is commonly found on plant glycoproteins (3). Cleavage with PNGase F will convert the asparagine residue to an aspartic residue, allowing identification of the glycosylation site by mass spectrometry (4). This purified enzyme is compatible with glycan analysis using mass spectrometry. |
| Accession # | P21163 N-terminal Se |
| 运输条件 | Blue Ice |
| 预期分子量 | 36 kDa |
| SDS-PAGE | 34 kDa, reducing conditions |
| 存放说明 | -80℃ |
| 纯度 | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| 参考文献 |
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RecombinantF. meningosepticum PNGase F (Catalog # 9109-GH) from R&D Systems and a leading competitor are able to deglycosylate 10 μg of RNase B at 37 °C in one hour. The E. coli-produced enzyme from R&D Systems offers a better value than the competition |
