| 货号 | AFL285 |
| 别名 | IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma | 全称 | Interferon gamma |
| 反应种属 | Human |
| 来源 | E. coli-derived Gln24-Gln166, with an N-terminal Met Based on MALDI-TOF mass spectrometric analysis, truncation of four amino acid residues from the C-terminus may be observed in the recombinant protein preparation. Produced using non-animal reagents in an animal-free laboratory. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is typically 0.15-0.75 ng/mL. The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586). |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile, deionized water. |
| 背景 | Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). |
| Accession # | CAA31639 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Met |
| 预期分子量 | 16.9 kDa |
| SDS-PAGE | 17 and 16 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
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