| 货号 | ACFP308 |
| 别名 | FL; Flt3 ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine | 全称 | fms-like Tyrosine Kinase 3 Ligand |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Thr27-Pro184 Produced in an animal component free process (ACFP). |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2323 (Human); 14256 (Mouse); 493796 (Feline) |
| 内毒素水平 | <0.01 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with mouse Flt-3. The ED50 for this effect is typically 0.2-1 ng/mL. The specific activity of recombinant human Flt-3 Ligand is approximately 1.2 x 103 U/μg, which is calibrated against recombinant human Flt-3 Ligand WHO Standard (NIBSC code: 96/532). |
| 溶解方法 | Reconstitute at 100 μg/mL in PBS. |
| 背景 | Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages (1-3). Mature human Flt‑3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail (4-7). Within the ECD, human Flt‑3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt‑3 Ligand, respectively. Human and mouse Flt‑3 Ligand show cross-species activity (4-6). Flt‑3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts (1, 8). Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates (8). Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain (4, 8). Alternate splicing of human Flt‑3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain (4, 5, 8). Both transmembrane and soluble Flt‑3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2 (3, 4, 6, 7). Flt‑3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation (2, 9). It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells (4-6). It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation (1, 10). Animal studies also show |
| Accession # | AAA17999.1 |
| 运输条件 | Blue Ice |
| N-terminal Sequence Analysis | Thr27 |
| 预期分子量 | 17.5 kDa |
| SDS-PAGE | 17-30 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
| 参考文献 |
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