Recombinant Human Activated Coagulation Factor Xa, ACFP (25 UG)
货号：
ACFP1063 基本售价：
11223.2 元 规格：
25 ug
产品信息
概述| 货号 | ACFP1063 |
| 别名 | Cf10; coagulation factor X; EC 3.4.21; EC 3.4.21.6; F10; factor Xa; FX; FXa; Prothrombinase; Stuart Factor; Stuart-Prower factor |
| 反应种属 | Human |
| 来源 | Spodoptera frugiperda,Sf 9 (baculovirus)-derived Met1-Lys488, with a C-terminal 10-His tag Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa. Produced in an animal component free process (ACFP). |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2159 (Human); 14058 (Mouse); 29243 (Rat) |
| 内毒素水平 | <1.0 EU per 1 μg of the protein by the LAL method. |
| 生物活性 | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2(Catalog #ES002). The specific activity is >800 pmol/min/μg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com. |
| 溶解方法 | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl and 5 mM CaCl |
| 背景 | As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41‑179) and heavy (residues 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF‑like domains and the heavy chain corresponds to the serine protease domain. The full-length human Factor X was expressed and the pro enzyme was purified and activated. The active protease (rhFactor Xa) was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. In addition to the activity described in the Activity Assay Protocol, rhFactor Xa also can be used to cleave fusion proteins containing a Factor Xa cleavage site. The conditions for the optimal cleavage of a particular fusion protein, such as the molar ratio between rhFXa and the protein and time and temperature of incubation, are protein-dependent and need to be individually determined. |
| Accession # | P00742 |
| 运输条件 | Blue Ice |
N-terminal
Sequence Analysis | Phe80, Tyr84, & Ile235 |
| 预期分子量 | 16.6 & 30 kDa |
| SDS-PAGE | 12-15 kDa and 30-36 kDa, reducing conditions |
| 存放说明 | -20℃ |
| 纯度 | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| 参考文献 | - Coagulation
- Interactive Pathway: Blood Coagulation Signaling Pathways
- Serine Proteases and Regulators
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