Recombinant Human FGF acidic, Animal-Free Protein (25 UG)【科瑞奥博】

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Recombinant Human FGF acidic, Animal-Free Protein (25 UG)

货号： AFL232-025 基本售价： 2706.6 元规格： 25 ug

产品信息

概述
货号	AFL232-025
别名	AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF1; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1
全称	Fibroblast Growth Factor acidic
反应种属	Human
来源	E. coli-derived Phe16-Asp155, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2246 (Human); 14164 (Mouse); 25317 (Rat); 281160 (Bovine)
内毒素水平	<0.01 EU per 1 μg of the protein by the LAL method.
生物活性	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is typically 0.1-0.3 ng/mL in the presence of 10 µg/mL of heparin.
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	FGF acidic, also known as FGF-1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%-33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species cross-reactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).
Accession #	NP_000791
运输条件	Blue Ice
N-terminal Sequence Analysis	Met
预期分子量	15.5 kDa
存放说明	-20℃
纯度	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
参考文献	Jaye, M. et al. (1986) Science 233:541. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159. Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073. Rajalingam, D. et al. (2007) Biochemistry 46:9225. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405. Imamura, T. et al. (1990) Science 249:1567. Wesche, J. et al. (2005) Biochemistry 44:6071. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245. Bouleau, S. et al. (2005) Oncogene 24:7839.

参考图片

1 μg/lane of Animal-Free^TM Recombinant Human FGF acidic (Catalog # AFL232) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.

Animal-Free^TM Recombinant Human FGF acidic (Catalog # AFL232) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED₅₀ for this effect is typically 0.1-0.3 ng/mL in the presence of 10 μg/mL heparin.