| 货号 | NBP2-36463PCP |
| 同种亚型 | IgG |
| 反应种属 | RbSpecies Glossary |
| 来源宿主 | Rabbit |
| 应用 | WB, ELISA, IHC, PAGE |
| 目标/特异性 | Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum. |
| 供应商 | Novus |
| 背景 | Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. |
| 存放说明 | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| 存储溶液 | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. |
| 浓度 | Please contact technical services for concentration. |
Western Blot: Rabbit IgG Isotype Control [NBP2-36463] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule (NBP2-36463). Lane 2: Rabbit IgG F(ab) Fragment (NBP1-97016). Lane 3: Rabbit IgG F(c) Fragment (NBP1-96784). Lane 4: Rabbit IgM Whole Molecule (NBP1-96968). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Blocked in blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins. (NBP1-72732) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in blocking buffer for 30 min at RT. Predicted/Obsevered Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher. | |
Flow (Intracellular): Rabbit IgG Isotype Control [NBP2-36463] - Intracellular staining for GLI1, GLI2 and GLI3 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using a secondary anti-rabbit IgG PE conjugated antibody. Image courtesy of Sherine Elsawa, Northern Illinois University. | |
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - SDS-Page of Rabbit IgG.Lane 1: Rabbit IgG - Non-Reduced.Lane 2: Rabbit IgG - Reduced.Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG.Other Band(s): None. | |
SDS-Page: Rabbit IgG Isotype Control [NBP2-36463] - Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5uL OPAL Pre-stained Marker. Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1ug per lane. Predicted/Observed size: Non-reduced at 150-170 kDa, Reduced at 55, 25 kDa. |
