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货号： bs-5973R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-5973R
- 英文名称
- SIRT7
- 中文名称
- 沉默调节样蛋白SirT7抗体
- 别 名
- NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7.
- Specific References (2) | bs-5973R has been referenced in 2 publications.[IF=1.11] Takumida, Masaya, Hiroshi Takumida, and Matti Anniko. "Localization of sirtuins in the mouse inner ear." Acta Oto-Laryngologica 0 (2014): 1-8. IHC-F ; Mouse.PubMed:24460154[IF=3.06] Wronska, A., et al. "Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats." Gerontology (2015). WB ;PubMed:26595207
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 心血管 免疫学 染色质和核信号 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶 结合蛋白 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 45kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human SIRT7:231-330/400
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
SIRT7 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Function:
NAD-dependent protein deacetylase. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex.
Subunit:
Interacts with UBTF and the RNA polymerase I complex. Interacts with histone H2A and/or histone H2B.
Subcellular Location:
Cytoplasm. Nucleus, nucleolus. Note=Located close to the nuclear membrane when in the cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.
Post-translational modifications:
Phosphorylated during mitosis (Probable).
Similarity:
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain.
SWISS:
Q9NRC8
Gene ID:
51547
Database links:Entrez Gene: 505662Cow
Entrez Gene: 51547Human
Entrez Gene: 209011Mouse
Omim: 606212Human
SwissProt: Q0P595Cow
SwissProt: Q9NRC8Human
SwissProt: Q8BKJ9Mouse
Unigene: 514636Human
Unigene: 292957Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:Small intestine (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Sample:Lymph node (Mouse)Lysate at 40 ug
Primary: Anti-SIRT7(bs-5973R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Tissue/cell: mouse placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SIRT7 Polyclonal Antibody, Unconjugated(bs-5973R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: mouse splenocytes(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).