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货号： bs-5791R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-5791R
- 英文名称
- Integrin Alpha V + Beta 6
- 中文名称
- 整合素αVβ6抗体
- 别 名
- CD51; Integrin alpha-V light chain; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); Integrin, alpha V; Integrin, beta 6; ITAV_HUMAN; ITGAV; ITGB6; Vitronectin receptor subunit alpha.
- Specific References (2) | bs-5791R has been referenced in 2 publications.[IF=8.31] Gao, Duo, et al."A near-infrared phthalocyanine dye-labeled agent for integrin avb6-targeted theranostics of pancreatic cancer." Biomaterials 53 (2015):229-238. IHC-P ; Human.PubMed:25890722[IF=3.02] Li, Haixia, et al. "Scutellarin inhibits the growth and invasion of human tongue squamous carcinoma through the inhibition of matrix metalloproteinase-2 and-9 and αvβ6 integrin." International Journal of Oncology 42.5 (2013): 1674-1681. IHC-P ; Mouse.PubMed:23546449
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 心血管 细胞生物 免疫学 信号转导 生长因子和激素 细胞粘附分子 细胞骨架
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 18/95/113kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Integrin Alpha V + Beta 6:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha-v beta-6 is upregulated in cancers and during tissue remodelling but is absent from resting adult tissues. Integrin alpha-v beta-6 promotes invasion and correlates with poor survival and therefore makes a promising therapeutic target.
Function:
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposis sarcoma lesions.
Subunit:
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat. Alpha-V/beta-6 binds to foot-and-mouth disease virus (FMDV) VP1 protein and acts as a receptor for this virus (By similarity). Alpha-V/beta-6 binds to coxsackievirus A9 and coxsackievirus B1 capsid proteins and acts as a receptor for these viruses. Interacts with RAB25.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
SWISS:
P06756
Gene ID:
3685
Database links:Entrez Gene: 3685 Human
Omim: 193210 Human
SwissProt: P06756 Human
Unigene: 436873 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-Integrin Alpha V+Beta 6(bs-5791R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 18/95/113kD
Observed band size: 113kD
Sample:Siha Cell (Human) Lysate at 40 ug
Primary: Anti-Integrin Alpha V + Beta 6(bs-5791R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/95/113 kD
Observed band size: 95 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Integrin Alpha V + Beta 6 (bs-5791R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18/95/113 kD
Observed band size: 95 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Integrin Alpha V+Beta 6 Polyclonal Antibody, Unconjugated(bs-5791R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control(blue): Hela (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice).
Primary Antibody:Rabbit Anti-Integrin Alpha V + Beta 6 antibody(bs-5791R), Dilution:1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.