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货号： bs-10560R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-10560R
- 英文名称
- FBXO42
- 中文名称
- FBXO42蛋白抗体
- 别 名
- F box only protein 42; F box protein 42; FBX42; FBXO 42; KIAA1332; FBX42_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 78kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human FBXO42:621-717/717
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
FBXO42 (F-box only protein 42) is a 717 amino acid protein that contains four Kelch repeats and one N-terminal F-box domain, and belongs to the F-box family of proteins. F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune response, signaling cascades and developmental processes) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkB-a and b-catenin, for degradation by the proteasome after ubiquitination.
Function:
Substrate-recognition component of some SCF(SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.Specifically recognizes p53/TP53, promoting its ubiquitination anddegradation.
Subunit:
Component of some SCF complex, composed of CUL1, SKP1,RBX1 and FBXO42. Interacts (via the kelch domain) with p53/TP53;interaction is direct.
Tissue Specificity:
Expressed at moderate levels in liver, ovary, kidney, testis and adult brain, and at low levels in heart and fetal brain, FBXO42 directly interacts with Skp1 p19 and CUL-1.
Similarity:
Contains 1 F-box domain.
Contains 4 Kelch repeats.
SWISS:
Q6P3S6
Gene ID:
54455
Database links:Entrez Gene: 54455 Human
Omim: 609109 Human
SwissProt: Q6P3S6 Human
Unigene: 522384 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FBXO42 Polyclonal Antibody, Unconjugated(bs-10560R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining