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货号： bs-3511R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3511R
- 英文名称
- FCGR1A/CD64
- 中文名称
- 免疫球蛋白G Fc段受体I
- 别 名
- CD 64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ia receptor; F Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for; Fc fragment of IgG, high affinity Ib, receptor (CD64); Fc fragment of IgG, high affinity Ib, receptor for; Fc fragment of IgG, high affinity Ic, receptor (CD64); Fc fragment of IgG, high affinity Ic, receptor for; Fc gamma receptor; Fc gamma receptor I A1; Fc gamma receptor I; Fc gamma receptor I B2; Fc gamma RI; Fc of IgG high affinity Ia receptor; Fc-gamma RIC; FCG 1; FCG1; FcgammaRI; FCGR 1; FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FCRIC; IGFR 1; IGFR1; IGFRB; IGFRC; IgG Fc receptor I; IgG Fc receptor IC; Immunoglobulin G Fc receptor I; Immunoglobulin G Fc receptor IB; Immunoglobulin G Fc receptor IC.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 细胞膜受体 细胞表面分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 41kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CD64/IGFR1:101-200/374 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The high affinity receptor for immunoglobulin G CD64 is encoded by a family of 3 genes that share over 98% of DNA sequence homology: FCGR1A, FCGR1B and FCGR1C. The FCGR1C gene, like FCGR1A, is located on 1q21. The third gene of this family, FCGR1B, was found at 1p12. CD64 plays a pivotal role in the immune response. It is a glycoprotein (MW 72 kD) that is constitutively expressed on human monocytes and macrophages.
Subunit:
Interacts with FCERG1; forms a functional signaling complex. Interacts with FLNA; prevents FCGR1A degradation. Interacts with EPB41L2, LAT and PPL. Interacts with HCK and LYN.
Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Stabilized at the cell membrane through interaction with FCER1G.
Tissue Specificity:
Monocyte/macrophage specific.
Similarity:
Belongs to the immunoglobulin superfamily. FCGR1 family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
P12314
Gene ID:
2209
Database links:Entrez Gene: 2209 Human
Omim: 146760 Human
SwissProt: P12314 Human
Unigene: 77424 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CD64是一种糖蛋白，主要定位于单核细胞和巨噬细胞,参与免疫应答反应。
- 产品图片
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-FCGR1A (bs-3511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Blank control: Mouse spleen cells(blue).
Primary Antibody: Rabbit Anti- FCGR1A/CD64 antibody(bs-3511R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
Primary antibody (bs-3511R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control: 293T Cells(blue).
Primary Antibody: Rabbit Anti-FCGR1A/CD64/AF647 Conjugated antibody (bs-3511R-AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD64 antibody(bs-3511R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.