Anti-Phospho-PERK(Thr980)【科瑞奥博】

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Anti-Phospho-PERK(Thr980)

货号： bs-3330R 基本售价： 1580.0 元规格： 100ul

产品信息

产品编号: bs-3330R

英文名称: Phospho-PERK (Thr980)

中文名称: 磷酸化蛋白激酶样内质网激酶抗体

别名: p-PERK(Thr980); PERK(Phospho Thr980); PERK(Phospho T980); HRI; HsPEK; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; WRS; DKFZp781H1925; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Heme regulated EIF2 alpha kinase.

: Specific References  (8)     |     bs-3330R has been referenced in 8 publications.
[IF=5.27] Kucuksayan, Ertan, et al. "Neutral Sphingomyelinase Inhibition Decreases ER Stress-Mediated Apoptosis and Inducible Nitric Oxide Synthase in Retinal Pigment Epithelial Cells." Free Radical Biology and Medicine (2014).  WB ;  Human.
PubMed:24742815
[IF=2.33] He, Yihuai, et al. "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." Molecular and Cellular Biochemistry (2014): 1-8.  WB ;  Human.
PubMed:24390087
[IF=3.63] Aslan, Mutay, et al. "Inhibition of Neutral Sphingomyelinase Decreases Elevated Levels of Inducible Nitric Oxide Synthase and Apoptotic Cell Death in Ocular Hypertensive Rats." Toxicology and Applied Pharmacology (2014).  WB ;  Rat.
PubMed:25201535
[IF=4.19] Xu, Demei, et al. "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response and calcium release." Chemical Research in Toxicology (2015).  WB ;  Human.
PubMed:25950987
[IF=0.00] Wang, Yu, et al. "Tanshinone II A Relieves Adriamycin-induced Myocardial Injury in Rat Model." International Journal of Chemistry 8.1 (2016): 40.  WB ;  Rat.
PubMed:not posted yet
[IF=4.65] Yu, H., et al. "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro."Cellular Physiology and Biochemistry 39.1 (2016): 123-136.  WB ;  Rat.
PubMed:27322831
[IF=4.94] Carmosino, Monica, et al. "The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling." Journal of Cellular and Molecular Medicine (2016).  WB, IF(ICC) ;  Human.
PubMed:27421120
[IF=0.00] Yan, Jiting, et al. "Catalpol prevents alteration of cholesterol homeostasis in non-alcoholic fatty liver disease via attenuating endoplasmic reticulum stress and NOX4 over-expression." RSC Advances 7.2 (2017): 1161-1176.  WB ;  Human.
PubMed:0

规格价格: 100ul/1580元购买大包装/询价

说明书: 100ul

产品类型: 磷酸化抗体

研究领域: 肿瘤免疫学信号转导细胞凋亡转录调节因子激酶和磷酸酶

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 119kDa

细胞定位: 细胞浆细胞膜

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthesised phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980:H(p-T)GQ

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].

Function:
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.

Subunit:
Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3.

Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Tissue Specificity:
Ubiquitous.

Post-translational modifications:
Autophosphorylated.
N-glycosylated.

Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 1 protein kinase domain.

SWISS:
Q9Z2B5

Gene ID:
13666

Database links:

Entrez Gene: 9451 Human

Entrez Gene: 13666 Mouse

Entrez Gene: 29702 Rat

Omim: 604032 Human

SwissProt: Q9NZJ5 Human

SwissProt: Q9Z2B5 Mouse

SwissProt: Q9Z1Z1 Rat

Unigene: 591589 Human

Unigene: 247167 Mouse

Unigene: 24897 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample:
Hela-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-PERK(Thr980) (bs-3330R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 119 kD
Observed band size: 119 kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PERK(Thr980)) Polyclonal Antibody, Unconjugated ( bs-3330R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (Black line): A431 (Black).
Primary Antibody (green line): Rabbit Anti-PERK(Thr980) antibody (bs-3330R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-PERK(Thr980) antibody (bs-3330R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.