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货号： bs-0426R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0426R
- 英文名称
- Myelin-oligodendrocyte glycoprotein
- 中文名称
- 髓鞘少树突胶质细胞糖蛋白抗体
- 别 名
- MOG(35-55); myelin oligo-dendrocyte glycoprotein-MOG; MGC26137; MOG alpha 6; MOG; MOGIG2; Myelin oligodendrocyte glycoprotein; MOG_HUMAN.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 神经生物学 信号转导 干细胞 细胞凋亡 细胞粘附分子 细胞膜蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 24kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse MOG:35-55/247 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Myelin oligodendrocyte glycoprotein (MOG) is a key CNS-specific autoantigen for primary demyelination in multiple sclerosis. Although the disease-inducing role of MOG has been established, its precise function in the CNS remains obscure. MOG is a type I integral membrane protein possessing a single extracellular Ig variable domain (Ig-V) (3, 13, 14). The amino acid sequence of MOG is highly conserved among animal species (>90%), indicative of an important biological function. MOG is specifically expressed in the CNS on the outermost lamellae of the myelin sheath as well as the cell body and processes of oligodendrocytes. The developmentally late expression of MOG correlates with the later stages of myelinogenesis, suggesting that MOG has a role in the completion, compaction, and/or maintenance of myelin, further suggesting that MOG has an adhesive function within the CNS . Consistent with MOGs possible adhesive role in the CNS, a homodimeric form of MOG has not only been observed after isolation from the CNS but has additionally been observed in situ.
Function:
Mediates homophilic cell-cell adhesion. Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication.
Subunit:
Homodimer. May form heterodimers between the different isoforms.
Subcellular Location:
Cell membrane; Multi-pass membrane protein (Potential).
Tissue Specificity:
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes.
DISEASE:
Defects in MOG are the cause of narcolepsy type 7 (NRCLP7) [MIM:614250]. Neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.
Similarity:
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
Q61885
Gene ID:
4340
Database links:Entrez Gene: 4340 Human
Entrez Gene: 17441 Mouse
Entrez Gene: 24558 Rat
Omim: 159465 Human
SwissProt: Q16653 Human
SwissProt: Q61885 Mouse
SwissProt: Q63345 Rat
Unigene: 141308 Human
Unigene: 210857 Mouse
Unigene: 9687 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
该蛋白与脊髓多发性硬化有直接关系。
有学者认为：用免疫组织化学方法所显示轴突周围少树突胶质细胞糖蛋白的异常先于髓鞘破坏。主张病变主要在少树突胶质细胞的超微结构方面，这方面还有待于深入研究。
- 产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Myelin-oligodendrocyte glycoprotein (bs-0426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- Myelin-oligodendrocyte glycoprotein (bs-0426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
Sample: Brain (Mouse) Lysate at 30 ug
Primary: Anti-Myelin-oligodendrocyte glycoprotein (bs-0426R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24 kD
Observed band size: 26 kD
Protein: brain(mouse)lysate at 30ug
Primary: Anti-alpha-SMA (bs-0426R) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000;
ECL excitated the fluorescence;
Predicted band size : 28kD
Observed band size : 22kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MOG) Polyclonal Antibody, Unconjugated (bs-0426R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37← for 20 min;
Incubation: Anti- MOG Polyclonal Antibody, Unconjugated(bs-0426R) 1:200, overnight at 4⒉C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining