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货号： bs-3012R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-3012R
- 英文名称
- phospho-Bim (Ser87)
- 中文名称
- 磷酸化细胞死亡调解子抗体
- 别 名
- BCL2 like 11; Apoptosis facilitator;BAM; Bcl 2 interacting mediator of cell death; Bcl 2 interacting protein Bim; Bcl 2 like protein 11; Bcl 2 related ovarian death agonis; Bcl 2 related ovarian death agonist; BCL2 interacting mediator of cell death; BCL2 interacting protein Bim; BCL2 like 11 (apoptosis facilitator); BCL2 like 11; BCL2 like 11 apoptosis facilitator; BCL2 like protein 11; BCL2 related ovarian death agonist; BCL2L11; BIM alpha3; BIM alpha4; BIM alpha5; BIM alpha6; BIM; BIM beta6; BIM beta7; BimEL; BimL; BOD; B2L11_HUMAN.
- Specific References (1) | bs-3012R has been referenced in 1 publications.[IF=4.65] Gogada, Raghu, et al. "Bim, a Proapoptotic Protein, Up-regulated via Transcription Factor E2F1-dependent Mechanism, Functions as a Prosurvival Molecule in Cancer." Journal of Biological Chemistry 288.1 (2013): 368-381.PubMed:23152504
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 22kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human Bim around the phosphorylation site of Ser87:RS(p-S)LL
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.
Function:
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit:
Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK.
Subcellular Location:
Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimEL: Mitochondrion. Isoform BimL: Mitochondrion. Isoform BimS: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.
Tissue Specificity:
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
O43521
Gene ID:
10018
Database links:Entrez Gene: 10018 Human
Omim: 603827 Human
SwissProt: O43521 Human
Unigene: 469658 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Bim是Bcl-2家族BH3-only亚家族的成员,是一种重要的凋亡调节蛋白，具有助凋亡活性作用。Bim广泛表达于正常细胞,对防御自身免疫、维持造血细胞稳态有重要意义,并具有肿瘤抑制因子活性.Bim的生理作用与它调节细胞凋亡的功能有关。
- 产品图片
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-phospho-Bim(Ser87) (bs-3012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Bim(Ser87)) Polyclonal Antibody, Unconjugated (bs-3012R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-Bim Polyclonal Antibody, Unconjugated(bs-3012R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-Bim(Ser87) Polyclonal Antibody, Unconjugated(bs-3012R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining