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货号： bs-12400R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-12400R
- 英文名称
- NKAP
- 中文名称
- NFKB激活蛋白抗体
- 别 名
- NF-kappa-B-activating protein; Nkap; NKAP_HUMAN; Nuclear NF kappaB activating protein; Nuclear NF kappa B activating protein; NFkB activating protein.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 染色质和核信号 信号转导 干细胞 转录调节因子 淋巴细胞 t-淋巴细胞
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Horse, Rabbit, Sheep,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 47kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NKAP:321-415/415
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
NFκB, a pleiotropic transcription factor, is present in almost all cell types and is involved in many biological processes including inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFκB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFκB1/p105, NFκB1/p50, REL and NFκB2/p52. This complex is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NKAP (NF-κ-B-activating protein) is a 415 amino acid nuclear protein that regulates TNF and IL1-induced NFκB activation. As component of a DNA-binding complex, NKAP also functions as a transcriptional repressor that acts on NOTCH target genes. Loss of NKAP blocks the development of αβT- cells, suggesting that it is required for their maturation through repression of NOTCH genes.
Function:
Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediated signaling required for T cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter.
Subunit:
Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3.
Subcellular Location:
Nucleus.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Similarity:
Belongs to the UPF0396 family.
SWISS:
Q8N5F7
Gene ID:
79576
Database links:Entrez Gene: 79576 Human
Omim: 300766 Human
SwissProt: Q8N5F7 Human
Unigene: 522771 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NKAP Polyclonal Antibody, Unconjugated(bs-12400R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining