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货号： bs-12025R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-12025R
- 英文名称
- GPR116
- 中文名称
- G蛋白偶联受体116抗体
- 别 名
- DKFZp564O1923; FLJ90640; G protein coupled receptor 116; G protein coupled receptor 116; GP116_HUMAN; GPR116; Ig Hepta homolog; KIAA0758; KIAA0758; KPG_001; OTTHUMP00000016557; Probable G protein coupled receptor 116; Probable G-protein coupled receptor 116； GPCR GPR116.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 神经生物学 信号转导 细胞膜受体 G蛋白偶联受体 G蛋白信号
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Rabbit,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 147kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human G protein coupled receptor 116:501-600/1346 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G protein coupled receptors translate extracellular signals into intracellular signals (G protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. GPR116 (G protein-coupled receptor 116) is a 1,346 amino acid multi-pass membrane protein that contains one SEA domain, one GPS domain and three Ig-like domains and belongs to the GPR family. Existing as a dilsulfide-liked homodimer at the cell surface, GPR116 exists as multiple alternatively spliced isoforms and is thought to play a role in regulating and maintaining proper acid-base balance throughout the cell.
Function:
May have a role in the regulation of acid-base balance.
Subunit:
Exists as disulfide-linked dimers at the cell surface
Subcellular Location:
Cell membrane; Multi-pass membrane protein
Post-translational modifications:
Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.
Similarity:
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 1 GPS domain.
Contains 3 Ig-like (immunoglobulin-like) domains.
Contains 1 SEA domain.
SWISS:
Q8IZF2
Gene ID:
221395
Database links:Entrez Gene: 221395 Human
SwissProt: Q8IZF2 Human
Unigene: 362806 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Blank control (blue line): Hela (Black).
Primary Antibody (green line): Rabbit Anti-GPR116 antibody (bs-12025R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.