产品中心
当前位置：首页>产品中心Anti-AQP9
货号： bs-2060R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2060R
- 英文名称
- AQP9
- 中文名称
- 水通道蛋白-9抗体
- 别 名
- aquaporin Protein-9; Aquaporin 9; AQP 9; AQP9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1; Aquaporin-9; AQP-9; Aquaglyceroporin-9; AQP9_HUMAN.
- Specific References (2) | bs-2060R has been referenced in 2 publications.[IF=1.28] Zhou, Jue, et al. "An atractylodes macrocephala koidz extract alleviates hyperandrogenism of polycystic ovarian syndrome." Int J Clin Exp Med 9.2 (2016): 2758-2767. IHC-P ; Rat.PubMed:0[IF=1.21] Vicente‐Carrillo, A., et al. "Membrane Stress During Thawing Elicits Redistribution of Aquaporin 7 But Not of Aquaporin 9 in Boar Spermatozoa."Reproduction in Domestic Animals (2016). IF(ICC) ; Other Species.PubMed:27405395
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 32kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human AQP9:201-295/295 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.
Function:
Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen.
Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.
SWISS:
O43315
Gene ID:
366
Database links:Entrez Gene: 366Human
Omim: 602914Human
SwissProt: O43315Human
Unigene: 104624Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
br>AQP9是一种选择性水通透膜转运蛋白，属于主体内在蛋白家族的成员之一。AQP9不但对水具有通透性，而且对其它一些中性溶质也具有通透性。
- 产品图片
Sample:Intestinal(Mouse) lysate at 30 ug
Primary: Anti-AQP9 (bs-2060R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 32 kD
Observed band size : 32 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-AQP9 (bs-2060R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 32 kD
Sample:Liver(Mouse) lysate at 30 ug
Primary: Anti-AQP9 (bs-2060R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 32 kD
Observed band size : 32 kD
Blank control: A549(blue).
Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (bs-2060R /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-2060R/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.