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货号： bs-11531R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-11531R
- 英文名称
- NPW/Neuropeptide W
- 中文名称
- 神经肽W抗体
- 别 名
- hPPL8; L8; L8C; Neuropeptide W; PPL8; PPNPW; Preproprotein L8; NPW_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Cow,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 3kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Neuropeptide W:33-55/165
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
NPW is a 165 amino acid secreted protein that is cleaved into two chains: neuropeptide W-23 (also designated NPW23 or L8) and neuropeptide W-30 (also known as NPW30 or L8C). Both NPW neuropeptides activate G-protein coupled receptors in the central nervous system to enhance cortisol secretion. Highly expressed in lymphoblastic leukemia, colorectal adenocarcinoma, fetal kidney, trachea and substantia nigra, NPW is also found at low levels in placenta, ovary, testis and uterus. NPW functions in organization of neuroendocrine signals and is also thought to enhance food and water intake as well as stress responses. The gene encoding NPW maps to human chromosome 16p13.3.
Function:
NPW is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade. The preproprotein is translated using a non-AUG initiation codon that is inferred from analyses of the mouse ortholog.
Subcellular Location:
Secreted
Tissue Specificity:
Detected at high levels in the substantia nigra, fetal kidney and trachea; at lower levels in testis, uterus, ovary and placenta. Not detectable in many regions of the central nervous system. Also detected at high levels in lymphoblastic leukemia and colorectal adenocarcinoma.
Similarity:
Belongs to the neuropeptide B/W family.
SWISS:
Q8N729
Gene ID:
283869
Database links:Entrez Gene: 283869 Human
Omim: 607997 Human
SwissProt: Q8N729 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropeptide W) Polyclonal Antibody, Unconjugated (bs-11531R) at 1:400 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.