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货号: bs-1700R 基本售价: 380.0 元 规格: 20ul
- 规格:20ul
- 价格:380.00元
- 规格:50ul
- 价格:780.00元
- 规格:100ul
- 价格:1380.00元
- 规格:200ul
- 价格:2200.00元
产品信息
- 产品编号
- bs-1700R
- 英文名称
- Wnt3a
- 中文名称
- 信号通路Wnt3a抗体
- 别 名
- MGC119418; MGC119419; MGC119420; Protein Wnt 3a Precursor; Protein Wnt-3a; Wingless type MMTV integration site family member 3A; Wnt 3a; WNT3A protein.
- Specific References (4) | bs-1700R has been referenced in 4 publications.[IF=1.48] Gao, Peng, et al. "Salvianolic acid B improves bone marrow-derived mesenchymal stem cell differentiation into alveolar epithelial cells type I via Wnt signaling." Molecular Medicine Reports. other ;PubMed:25892295[IF=1.03] Lu, Juan, et al. "Expression of Wnt3a, Wnt10b, β-catenin and DKK1 in periodontium during orthodontic tooth movement in rats." Acta Odontologica Scandinavica (2015): 1-7. IHC-P ; Rat.PubMed:26414930[IF=2.37] Hayashi, Kouhei, et al. "Involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland." Biochemical and Biophysical Research Communications (2016). WB ; Mouse.PubMed:27369076[IF=13.12] Böscke, Robert, et al. "Wnt Signaling in Chronic Rhinosinusitis with Nasal Polyps." American Journal of Respiratory Cell and Molecular Biology ja (2017). IF(ICC) ; Human.PubMed:28059551
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 神经生物学 干细胞 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 37kDa
- 细胞定位
- 细胞外基质 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Wnt3a:281-352/352
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
Function:
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
Subunit:
Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6.
Subcellular Location:
Secreted, extracellular space, extracellular matr
Tissue Specificity:
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
Post-translational modifications:
Palmitoylation at Ser-209 by PORCN is required for proper trafficking to cell surface, vacuolar acidification is critical to release palmitoylated WNT3A from WLS in secretory vesicles.
Similarity:
Belongs to the Wnt family.
SWISS:
P56704
Gene ID:
89780
Database links:Entrez Gene: 89780 Human
Entrez Gene: 22416 Mouse
Entrez Gene: 303181 Rat
Omim: 606359 Human
SwissProt: P56704 Human
SwissProt: P27467 Mouse
Unigene: 336930 Human
Unigene: 1367 Mouse
Unigene: 130971 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Wnt3a是Wnt家族的成员之一,对神经干细胞的增殖及分化有一定的调控作用,Wnt3a蛋白可以促进神经干细胞向神经元分化.
- 产品图片
- Sample:
U2os Cell(Human)Lysate at 30 ug
Primary: Anti- Wnt3a (bs-1700R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kDSample:
Brain(Rat) lysate at 30ug;
Uterus(Mouse ) lysates, 30ug;
Primary: Anti-Wnt3a (bs-1700R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 37kD
Observed band size : 37kDImages provided the Independent Validation Program (badge number 029652)Formalin-fixed and paraffin embedded human small intestine labeled with Rabbit Anti-Wnt3a Polyclonal Antibody (bs-1700R) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Wnt3a Polyclonal Antibody, Unconjugated(bs-1700R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining