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货号： bs-2184R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2184R
- 英文名称
- NQO1
- 中文名称
- 醌氧化还原酶抗体
- 别 名
- Azoreductase; Cytochrome b 5 reductase; DHQU; DIA 4; DIA4; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase (NADH/NADPH); Diaphorase 4; Dioxin inducible 1; DT diaphorase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase quinone 1; NAD(P)H menadione oxidoreductase 1 dioxin inducible; NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR 1; NMOR I; NMOR1; NMORI; NQO 1; NQO1; NQO1_HUMAN; Phylloquinone reductase; Phylloquinone reductase; QR 1; QR1; Quinone reductase 1; Quinone reductase 1.
- Specific References (4) | bs-2184R has been referenced in 4 publications.[IF=3.83] Wang, Peng, et al. "Geraniin exerts cytoprotective effect against cellular oxidative stress by upregulation of Nrf2-mediated antioxidant enzymes expression via PI3K/AKT and ERK1/2 pathway." Biochimica et Biophysica Acta (BBA)-General Subjects (2015). WB ; Human.PubMed:25917210[IF=2.40] Zhao, Feng, et al. "Silymarin attenuates paraquat‐induced lung injury via Nrf2‐mediated pathway in vivo and in vitro." Clinical and Experimental Pharmacology and Physiology (2015). WB ; Human.PubMed:26173462[IF=1.07] Liu, Cui-Zhen, et al. "HBSP attenuates lipopolysaccharide-induced inflammatory response in human renal proximal tubular epithelial cells by induction of Nrf2 via PI3K/Akt pathway." Int J Clin Exp Med 10.1 (2017): 587-597. WB ; Human.PubMed:0[IF=2.55] Shen, Haitao, et al. "Chloroquine attenuates paraquat-induced lung injury in mice by altering inflammation, oxidative stress and fibrosis." International Immunopharmacology 46 (2017): 16-22. WB ; Mouse.PubMed:28249220
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 信号转导 合成与降解 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Pig, Horse, Rabbit, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 31kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NQO1:201-274/274
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This proteins enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimers disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq].
Function:
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Subunit:
Homodimer.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the NAD(P)H dehydrogenase (quinone) family.
SWISS:
P15559
Gene ID:
1728
Database links:Entrez Gene: 610935 Dog
Entrez Gene: 1728 Human
Entrez Gene: 18104 Mouse
Entrez Gene: 100286873 Pig
Entrez Gene: 24314 Rat
Omim: 125860 Human
SwissProt: P15559 Human
SwissProt: Q64669 Mouse
SwissProt: P05982 Rat
Unigene: 406515 Human
Unigene: 252 Mouse
Unigene: 11234 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
合成与降解（Synthesis and Degradation）
醌氧化还原酶（NQO1）是一种黄素酶，它催化醌双电子还原反应，以减少氧自由基的产生。经研究发现NQO1可能有抗氧化效应，目前多用于帕金森病（PD）和部分肿瘤方面的研究。
醌是一种有毒的化合物,能诱发哺乳动物细胞癌变、突变和坏死。
- 产品图片
Sample:
Lane1: Liver(Rat) Lysate at 30 ug
Lane2: Brain(Rat) Lysate at 30 ug
Primary: Anti-NQO1 (bs-2184R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 31kD
Observed band size : 30kD
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-NQO1 (bs-2184R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Protein: A549 lysate at 30ug;
Primary: Anti-NQO1 (bs-2184R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 31 kD
Observed band size :31 kD
Tissue/cell: mouse colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NQO1 Polyclonal Antibody, Unconjugated(bs-2184R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining