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货号： bs-1495R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1495R
- 英文名称
- OCLN
- 中文名称
- 紧密连接蛋白抗体
- 别 名
- Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.
- Specific References (6) | bs-1495R has been referenced in 6 publications.[IF=2.46] Pan, Li, et al. "Effects of Soybean Agglutinin on Mechanical Barrier Function and Tight Junction Protein Expression in Intestinal Epithelial Cells from Piglets." International Journal of Molecular Sciences 14.11 (2013): 21689-21704. Pig.PubMed:24189218[IF=2.03] Gu, Yan-ting, et al. "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." Neuroscience letters 493.3 (2011): 140-144. WB ; Rat.PubMed:21334421[IF=2.46] Zhao, Yuan, et al. "β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2." International Journal of Molecular Sciences 15.2 (2014): 1915-1926. Pig.PubMed:24473141[IF=2.73] Sun, Yan‐Na, et al. "Effects of insulin combined with idebenone on blood–brain barrier permeability in diabetic rats." Journal of Neuroscience Research (2014). WB ; Rat.PubMed:25421718[IF=2.71] Guo, Xiaoling, et al. "Matrigel and Activin A promote cell-cell contact and anti-apoptotic activity in cultured human retinal pigment epithelium cells." Experimental Eye Research (2016). IF(ICC) ; Human.PubMed:27130547[IF=2.86] Zhao, Yuan, et al. "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets."International journal of molecular sciences 12.12 (2011): 8502-8512. WB, IHC-P ; Pig.PubMed: 22272087
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 转录调节因子 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=3µg/Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 57kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse OCLN:431-522/522 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. [provided by RefSeq].
Function:
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Subunit:
Interacts with TJP1/ZO1 and with VAPA.
Subcellular Location:
Membrane; Multi-pass membrane protein. Cell junction, tight junction.
Tissue Specificity:
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR. Dephosphorylated by PTPRJ. May be phosphorylated by PKC during translocation to cell-cell contacts.
Similarity:
Belongs to the ELL/occludin family.
Contains 1 MARVEL domain.
SWISS:
Q61146
Gene ID:
18260
Database links:Entrez Gene: 18260 Mouse
Entrez Gene: 100506658 Human
Entrez Gene: 397236 Pig
Entrez Gene: 83497 Rat
Entrez Gene: 403844 Dog
Omim: 602876 Human
SwissProt: Q28269 Dog
SwissProt: Q16625 Human
SwissProt: Q61146 Mouse
SwissProt: Q6P6T5 Rat
Unigene: 592605 Human
Unigene: 4807 Mouse
Unigene: 31429 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Sample: A549 Cell Lysate at 30 ug
Primary: Anti- OCLN (bs-1495R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 60 kD
The blue histogram is unstained cells (293FT cells).
The Wathet Blue histogram is cells stained with secondary antibody alone.
The Orange histogram is cells stained with rabbit IgG isotype control antibodyplus secondary antibody.
The green histogram is cells stained with Rabbit Anti-OCLN antibody (bs-1495R) plus secondary antibody (Concentraion 1: 20).